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Image Search Results
Journal: Aging and Disease
Article Title: Human Adipose Mesenchymal Stem Cell-derived Exosomes Protect Mice from DSS-Induced Inflammatory Bowel Disease by Promoting Intestinal-stem-cell and Epithelial Regeneration
doi: 10.14336/AD.2021.0601
Figure Lengend Snippet: hADSC-Exo reshape the cell structure in colon crypts of DSS-induced mice IBD . ( A-D ) Immunofluorescence staining analysis of expression of epithelial cells proliferation related specific marker Ki67 and β-Catenin in mice colon tissue sections (scale bar=20 µm; n=7, per group). ( E-H ) Immuno-fluorescence staining analysis of expression of colon stem cells specific markers EPH B2 and LGR5 in mice colon tissue sections (scale bar=20 µm; n=7, per group). (I and J) Immuno-fluorescence staining analysis of expression of goblet cells specific marker Mucin in mice colon tissue sections (scale bar=20 µm; n=7, per group).
Article Snippet:
Techniques: Immunofluorescence, Staining, Expressing, Marker, Fluorescence
Journal: Aging and Disease
Article Title: Human Adipose Mesenchymal Stem Cell-derived Exosomes Protect Mice from DSS-Induced Inflammatory Bowel Disease by Promoting Intestinal-stem-cell and Epithelial Regeneration
doi: 10.14336/AD.2021.0601
Figure Lengend Snippet: hADSC-Exo up-taken by Hcoepic cells and promotes wound healing of Hcoepic cells . ( A ) Uptake of hADSC-Exo by Hcoepic cells at 4, 12, 24, 48 h after co-culture. ( B ) Mean fluorescence intensity (MFI) of Dil in Hcoepic cells. (C and D) hADSC-Exo promotes the migratory activity of Hcoepic cells and accelerate wound healing, at 0, 4, 6, 12, 24, 48 h after co-culture.
Article Snippet:
Techniques: Co-Culture Assay, Fluorescence, Activity Assay
Journal: Aging and Disease
Article Title: Human Adipose Mesenchymal Stem Cell-derived Exosomes Protect Mice from DSS-Induced Inflammatory Bowel Disease by Promoting Intestinal-stem-cell and Epithelial Regeneration
doi: 10.14336/AD.2021.0601
Figure Lengend Snippet: hADSC-Exo facilitates Hcoepic cells proliferation, promotes cell cycle and inhibits cell apoptosis . (A and B) CCK8 assay analysis the exosomes’ influences on the proliferation of Hcoepic cells at 24 (A) & 48 h (B). exosome concentration: 0, 0.1, 2, 5, 8, 10 µg/ml. ( C ) Exosomes’ influences on cell cycle of Hcoepic cells after hADSC-Exo administration 48 h at exosome concentration: 200 ug/ml. ( D ) Exosomes’ influences on cell apoptosis of Hcoepic cells after hADSC-Exo administration 48 h at exosome concentration: 200 µg/ml (All experiments repeated at least 3 times).
Article Snippet:
Techniques: CCK-8 Assay, Concentration Assay